Development of Two Functional Markers of <i>Badh2</i> Gene in Guangxi Fragrant Rice

Fragrance in rice is one of the most important quality traits, which resulted from loss function betaine aldehyde dehydrogenase ( Badh2 ) gene on chromosome 8. The mutation of   leads to accumulation 2-acetyl-1-pyrroline (2-AP), known as main volatile fragrant rice. At least 18 allelic variations have been identified in   genes Marker assisted selection has proved be an effective way breeding. Traditional marker detection methods, such Sanger sequencing or SSR molecular marker, are found low efficient. To develop a more dynamic method, we adopt Real-time PCR method detect common site Guangxi. In this study,   gene 40 accessions collected Guangxi province was sequenced. Most belonged 806 bp deletion between exon 4-5 badh2-E4-5.1 and 8 7 badh2-E7 ). Two SNP markers were developed, used verify sequenced accessions. 93.75% results using consistent with sequencing. Further, 50 local varieties examined by PCR. A total 24 carry  badh-E4-5  allele 22 detected with  badh-E7 . two functional developed useful These will improve efficiency